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anti fn1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti fn1
    Anti Fn1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fn1/Fibronectin%2FFN1+Rabbit+mAb/pmc12925212-498-62-66
    Average 96 stars, based on 241 article reviews
    anti fn1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Targeting FN1 to overcome gemcitabine resistance in gallbladder cancer: Mechanistic insights and an iRGD-modified PEG-PLGA nanoparticle delivery strategy
    Article Snippet: Equal amounts of protein (50 μg per lane) were resolved by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (FFP28, Beyotime, Shanghai, China). .. The membranes were blocked in 5% non-fat milk at ambient temperature for 1 h and incubated overnight at 4 °C with primary antibodies including anti-dCK (rabbit; 1:1000, 17758-1-AP, Proteintech, Wuhan, China), anti-hENT1 (rabbit; 1:5000, 29862-1-AP, Proteintech, Wuhan, China), anti-PI3K (rabbit; 1:1000, ab191606, Abcam, UK), anti-p-PI3K (rabbit; 1:1000, ab278545, Abcam, UK), anti-AKT (rabbit; 1:1000, 4691, CST, USA), anti-p-AKT (rabbit; 1:2000, 4060, CST, USA), anti-FN1 (rabbit; 1:1000, 26836, CST, USA), and anti-β-actin (rabbit; 1:1000, ab8226, Abcam, UK), β-actin served as a loading control. .. After washing with PBS containing Tween 20 (PBST), the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit/mouse IgG (1:10,000, BA1054/BA1050, Boster, Wuhan, China) for 1 h at ambient temperature, followed by six 5-min washes with PBST.

    Control:

    Article Title: Targeting FN1 to overcome gemcitabine resistance in gallbladder cancer: Mechanistic insights and an iRGD-modified PEG-PLGA nanoparticle delivery strategy
    Article Snippet: Equal amounts of protein (50 μg per lane) were resolved by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (FFP28, Beyotime, Shanghai, China). .. The membranes were blocked in 5% non-fat milk at ambient temperature for 1 h and incubated overnight at 4 °C with primary antibodies including anti-dCK (rabbit; 1:1000, 17758-1-AP, Proteintech, Wuhan, China), anti-hENT1 (rabbit; 1:5000, 29862-1-AP, Proteintech, Wuhan, China), anti-PI3K (rabbit; 1:1000, ab191606, Abcam, UK), anti-p-PI3K (rabbit; 1:1000, ab278545, Abcam, UK), anti-AKT (rabbit; 1:1000, 4691, CST, USA), anti-p-AKT (rabbit; 1:2000, 4060, CST, USA), anti-FN1 (rabbit; 1:1000, 26836, CST, USA), and anti-β-actin (rabbit; 1:1000, ab8226, Abcam, UK), β-actin served as a loading control. .. After washing with PBS containing Tween 20 (PBST), the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit/mouse IgG (1:10,000, BA1054/BA1050, Boster, Wuhan, China) for 1 h at ambient temperature, followed by six 5-min washes with PBST.

    Western Blot:

    Article Title: Precision Synthesis and Antiliver Fibrosis Activity of a Highly Branched Acidic 63-Mer Pectin Polysaccharide.
    Article Snippet: .. Anti-FN1 (#26836S, cell signaling technology), anti-collagen (bs-10423R, Bioss), anti-αSMA (A17910, ABclonal), anti-GAPDH antibody (AC002, ABclonal) was used in the Western blot test. ..

    Article Title: MYBL2 accelerates epithelial-mesenchymal transition and hepatoblastoma metastasis via the Smad/SNAI1 pathway
    Article Snippet: .. Western blotting Anti-MYBL2 (Abcam-12296, 1:2500), anti-ZO-1 (Invitrogen-33-9100, 1:1000), anti-GAPDH (Proteintech-60004, 1:5000), anti-FN1 (Cell Signaling-26836, 1:1000), anti-AKT (Cell Signaling-9272, 1:1000), anti-phospho-Akt (Ser473) (Cell Signaling-4060, 1:2000), anti-GSK-3β (Cell Signaling-9832, 1:1000), anti-phospho-GSK-3β (Ser9) (Cell Signaling-9323, 1:1000), anti-Smad2/3 (Cell Signaling-8685, 1:1000), anti-phospho-Smad2/3 (Cell Signaling-8828, 1:1000), anti-ERK1/2 (Cell Signaling-9102, 1:1000), anti-phospho-ERK1/2 (Cell Signaling-4370, 1:2000), and anti-SNAI1 (Abcam-216347, 1:1000) primary antibodies were used. .. HRP-linked anti-IgG (Cell Signaling, 1:5000) was used as the secondary antibody.

    Article Title: PFKFB3 facilitates cell proliferation and migration in anaplastic thyroid carcinoma via the WNT/β-catenin signaling pathway.
    Article Snippet: Purpose Despite the involvement of 6‐phosphofructo‐2‐kinase/fructose‐2,6‐biphosphatase3 (PFKFB3) in the proliferation and metastasis of diverse tumor types, its biological functions and related molecular mechanisms in anaplastic thyroid carcinoma (ATC) remain largely unclear.. Methods Datasets from the Gene Expression Omnibus, the Cancer Genome Atlas and immunohistochemistry (IHC) analyses were employed to measure the expression level of PFKFB3 in ATC.. A series of assays were performed to analyze the role of PFKFB3 and its inhibitor KAN0438757 in ATC cell proliferation and migration.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response.
    Article Snippet: Background: Pneumocystis carinii is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.. Aims: This study attempted to investigate the role of P. carinii infection-related genes in regulating lung fibrosis in mice.. Methods: A screening of P. carinii infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain P. carinii infection-related key genes.

    Coagulation:

    Article Title: Pneumocystis carinii infection drives upregulation of Fn1 expression that causes pulmonary fibrosis with an inflammatory response.
    Article Snippet: Background: Pneumocystis carinii is an opportunistic fungal pathogen that may cause pneumonia and lead to pulmonary fibrosis.. Aims: This study attempted to investigate the role of P. carinii infection-related genes in regulating lung fibrosis in mice.. Methods: A screening of P. carinii infection-related differential mRNAs was performed using the GEO database, followed by protein–protein interaction (PPI) network construction using the STRING website in order to obtain P. carinii infection-related key genes.



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